Journal: iScience

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager

doi: 10.1016/j.isci.2024.111289

Figure Lengend Snippet: Target binding, Vγ9Vδ2 T cell activation, and tumor cell lysis by the PSMA-Vδ2-Fc bsTCE (A) PSMA-Vδ2-Fc bsTCE and control bsTCE binding to expanded Vγ9Vδ2 T cells ( n = 6), LNCaP (left: n = 3, right: n = 2), and PC3 cells ( n = 1). (B) PSMA expression by 22Rv1 and VCaP shown as histograms. (C) Vγ9Vδ2 T cell CD107a expression, CD25 expression, and IFN-γ secretion ( n = 3). (D) Target cell lysis ( n = 3–4) in 24 h co-cultures of expanded Vγ9Vδ2 T cells with 22Rv1, VCaP, LNCaP (WT or PSMA KO), or PC3 with indicated concentrations of bsTCE. (E) Vγ9Vδ2 T cell CD25 expression ( n = 3) and target cell lysis ( n = 3) in 24 h co-cultures of expanded Vγ9Vδ2 T cells with 22Rv1, VCaP, LNCaP, or PC3 with 100 nM PSMA-Vδ2-Fc bsTCE or control bsTCEs. Data were generated using flow cytometry (A left, B, C; CD107a and CD25, D, E), ELISA (A, middle and right) or CBA (C, IFNγ). Data represent mean ± S.E.M.

Article Snippet: LNCaP (PSMA + clone FGC, CRL-1740) and PC3 (PSMA − , CRL-1435) were obtained from American Type Culture Collection (ATCC) and VCaP (PSMA + , 06020201-1VL) and 22Rv1 (PSMA + , 5092802) from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Binding Assay, Activation Assay, Lysis, Control, Expressing, Generated, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager

doi: 10.1016/j.isci.2024.111289

Figure Lengend Snippet: Impact of DNAM-1 and NKG2D receptor interactions in PSMA-Vδ2-Fc bsTCE mediated Vγ9Vδ2 T cell activation and prostate tumor cell lysis (A) CD107a expression (4 h culture, n = 4) and (B) tumor cell lysis (24 h culture, n = 4) from cultures of expanded Vγ9Vδ2 T cells pre-incubated with 10 μg/mL anti-Fc receptor Ab and α-DNAM-1 blocking antibody or IgG1 isotype control with LNCaP, VCaP, or 22Rv1 tumor cells with indicated concentrations of PSMA-Vδ2-Fc bsTCE. (C and D) (C) CD107a expression (4 h culture, n = 4) and (D) tumor cell lysis (24 h culture, n = 4) from cultures of expanded Vγ9Vδ2 T cells pre-incubated with 10 μg/mL anti-Fc receptor Ab and α-NKG2D blocking antibody or IgG1 isotype control with LNCaP, VCaP, or 22Rv1 tumor cells with indicated concentrations of PSMA-Vδ2-Fc bsTCE. (E) CD107a expression on Vγ9Vδ2 T cells derived from malignant prostate tissue ( n = 3) of PCa patients after 4 h co-culture with 0.05 nM PSMA-Vδ2-Fc bsTCE and α-DNAM-1 or α-NKG2D blocking Ab. Data were all generated using flow cytometry and circles represent individual data-points. Box and whisker plots indicate the median, 25th to 75th percentiles and minimum to maximum and bar graphs represent mean ± S.E.M. Paired t test (A, C–E). p < 0.05: ∗, p < 0.01: ∗∗ and p < 0.001: ∗∗∗.

Article Snippet: LNCaP (PSMA + clone FGC, CRL-1740) and PC3 (PSMA − , CRL-1435) were obtained from American Type Culture Collection (ATCC) and VCaP (PSMA + , 06020201-1VL) and 22Rv1 (PSMA + , 5092802) from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Activation Assay, Lysis, Expressing, Incubation, Blocking Assay, Control, Derivative Assay, Co-Culture Assay, Generated, Flow Cytometry, Whisker Assay

Journal: Molecular and cellular endocrinology

Article Title: Adrenal Androgens Rescue Prostatic Dihydrotestosterone Production and Growth of Prostate Cancer Cells after Castration

doi: 10.1016/j.mce.2019.02.018

Figure Lengend Snippet: AR-dependent stimulation of growth by adrenal androgens. (A) DHEAS and DHEA stimulated growth of VCaP cells. (B) DHEAS (DS) stimulated growth was diminished by AR antagonist bicalutamide (Bica) and STX64 (B). Cells were treated for 3 days and 7 days. Growth was assessed using MTT assay. Data were presented in percentage to untreated controls at each time point (day). * p < 0.05 compared to control. # p < 0.05 compared to DHEAS.

Article Snippet: The mice were inoculated subcutaneously with 2 × 10 6 VCaP cells suspended in 0.1 mL Matrigel (Cat#356237, Corning Life Sciences, Corning, NY) 3 days after T-silastic tubing implantation.

Techniques: MTT Assay, Control

Journal: eLife

Article Title: circFL-seq reveals full-length circular RNAs with rolling circular reverse transcription and nanopore sequencing

doi: 10.7554/eLife.69457

Figure Lengend Snippet:

Article Snippet: Cell line ( H. sapiens ) , VCaP , iCell Bioscience , RRID: CVCL_2235 , .

Techniques: